paad cell lines capan–2 Search Results


paad cell lines  (ATCC)
99
ATCC paad cell lines
Analysis of circ_0041150 expression and its impact on <t>PAAD</t> cell proliferation. A The chromosomal location and structure of circ_0041150 were depicted. B The expression patterns of circ_0041150 <t>in</t> <t>HPDE6-C7</t> and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were examined by qRT-PCR ( n = 4). C , D The stability of circ_0041150, compared to its host gene RPH3AL mRNA, was analyzed by qRT-PCR after RNase R digestion ( n = 4). E , F Its subcellular localization was assessed via qRT-PCR ( n = 4). G BxPC-3 cells were transfected with sh-circ_0041150#1 or sh-circ_0041150#2, and circ_0041150 expression was detected by qRT-PCR ( n = 4). H , I Cell proliferation in BxPC-3 cells with circ_0041150 knockdown was evaluated by CCK-8 and EdU incorporation assays ( n = 4). J PANC-1 cells were transfected with p-circ_0041150, and circ_0041150 expression was detected by qRT-PCR ( n = 4). K , L Cell proliferation in PANC-1 cells overexpressing circ_0041150 was assessed by CCK-8 and EdU incorporation assays ( n = 4). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control
Paad Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paad+cell+lines+capan%E2%80%932/BxPC-3/pmc12972392-70-9-19
Average 99 stars, based on 1 article reviews
paad cell lines - by Bioz Stars, 2026-09
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human paad cell lines capan 2  (ATCC)
97
ATCC human paad cell lines capan 2
Analysis of circ_0041150 expression and its impact on <t>PAAD</t> cell proliferation. A The chromosomal location and structure of circ_0041150 were depicted. B The expression patterns of circ_0041150 <t>in</t> <t>HPDE6-C7</t> and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were examined by qRT-PCR ( n = 4). C , D The stability of circ_0041150, compared to its host gene RPH3AL mRNA, was analyzed by qRT-PCR after RNase R digestion ( n = 4). E , F Its subcellular localization was assessed via qRT-PCR ( n = 4). G BxPC-3 cells were transfected with sh-circ_0041150#1 or sh-circ_0041150#2, and circ_0041150 expression was detected by qRT-PCR ( n = 4). H , I Cell proliferation in BxPC-3 cells with circ_0041150 knockdown was evaluated by CCK-8 and EdU incorporation assays ( n = 4). J PANC-1 cells were transfected with p-circ_0041150, and circ_0041150 expression was detected by qRT-PCR ( n = 4). K , L Cell proliferation in PANC-1 cells overexpressing circ_0041150 was assessed by CCK-8 and EdU incorporation assays ( n = 4). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control
Human Paad Cell Lines Capan 2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paad+cell+lines+capan%E2%80%932/Capan-2/pmc08185698-39-1-8
Average 97 stars, based on 1 article reviews
human paad cell lines capan 2 - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier

Image Search Results


Analysis of circ_0041150 expression and its impact on PAAD cell proliferation. A The chromosomal location and structure of circ_0041150 were depicted. B The expression patterns of circ_0041150 in HPDE6-C7 and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were examined by qRT-PCR ( n = 4). C , D The stability of circ_0041150, compared to its host gene RPH3AL mRNA, was analyzed by qRT-PCR after RNase R digestion ( n = 4). E , F Its subcellular localization was assessed via qRT-PCR ( n = 4). G BxPC-3 cells were transfected with sh-circ_0041150#1 or sh-circ_0041150#2, and circ_0041150 expression was detected by qRT-PCR ( n = 4). H , I Cell proliferation in BxPC-3 cells with circ_0041150 knockdown was evaluated by CCK-8 and EdU incorporation assays ( n = 4). J PANC-1 cells were transfected with p-circ_0041150, and circ_0041150 expression was detected by qRT-PCR ( n = 4). K , L Cell proliferation in PANC-1 cells overexpressing circ_0041150 was assessed by CCK-8 and EdU incorporation assays ( n = 4). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Journal: Discover Oncology

Article Title: Circ_0041150 inhibits proliferation of pancreatic adenocarcinoma cells by regulating triglyceride accumulation via the miR-1178-3p/AADAC axis

doi: 10.1007/s12672-026-04577-1

Figure Lengend Snippet: Analysis of circ_0041150 expression and its impact on PAAD cell proliferation. A The chromosomal location and structure of circ_0041150 were depicted. B The expression patterns of circ_0041150 in HPDE6-C7 and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were examined by qRT-PCR ( n = 4). C , D The stability of circ_0041150, compared to its host gene RPH3AL mRNA, was analyzed by qRT-PCR after RNase R digestion ( n = 4). E , F Its subcellular localization was assessed via qRT-PCR ( n = 4). G BxPC-3 cells were transfected with sh-circ_0041150#1 or sh-circ_0041150#2, and circ_0041150 expression was detected by qRT-PCR ( n = 4). H , I Cell proliferation in BxPC-3 cells with circ_0041150 knockdown was evaluated by CCK-8 and EdU incorporation assays ( n = 4). J PANC-1 cells were transfected with p-circ_0041150, and circ_0041150 expression was detected by qRT-PCR ( n = 4). K , L Cell proliferation in PANC-1 cells overexpressing circ_0041150 was assessed by CCK-8 and EdU incorporation assays ( n = 4). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Article Snippet: The human pancreatic duct epithelial cell line (HPDE6-C7) and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were obtained from ATCC.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Knockdown, CCK-8 Assay, Two Tailed Test, Control

Effects of circ_0041150 on TG accumulation of PAAD cells. A Flutamide hydrolase activity was assayed in BxPC-3 cells following circ_0041150 knockdown ( n = 4). B , C Lipid deposition, induced by FFA, was visualized in BxPC-3 cells with knocked-down circ_0041150 via BODIPY 493/503 staining using fluorescence microscopy (40×; scale bar = 20 μm) ( n = 4). D Triglyceride (TG) content was quantified in BxPC-3 cells post-knockdown. E Flutamide hydrolase activity was assayed in PANC-1 cells following circ_0041150 overexpression ( n = 4). F , G FFA-induced lipid deposition was visualized in PANC-1 cells with overexpressed circ_0041150 via BODIPY 493/503 staining using fluorescence microscopy (40×; scale bar = 20 μm) ( n = 4). H TG content was quantified in PANC-1 cells post-overexpression ( n = 4). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Journal: Discover Oncology

Article Title: Circ_0041150 inhibits proliferation of pancreatic adenocarcinoma cells by regulating triglyceride accumulation via the miR-1178-3p/AADAC axis

doi: 10.1007/s12672-026-04577-1

Figure Lengend Snippet: Effects of circ_0041150 on TG accumulation of PAAD cells. A Flutamide hydrolase activity was assayed in BxPC-3 cells following circ_0041150 knockdown ( n = 4). B , C Lipid deposition, induced by FFA, was visualized in BxPC-3 cells with knocked-down circ_0041150 via BODIPY 493/503 staining using fluorescence microscopy (40×; scale bar = 20 μm) ( n = 4). D Triglyceride (TG) content was quantified in BxPC-3 cells post-knockdown. E Flutamide hydrolase activity was assayed in PANC-1 cells following circ_0041150 overexpression ( n = 4). F , G FFA-induced lipid deposition was visualized in PANC-1 cells with overexpressed circ_0041150 via BODIPY 493/503 staining using fluorescence microscopy (40×; scale bar = 20 μm) ( n = 4). H TG content was quantified in PANC-1 cells post-overexpression ( n = 4). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Article Snippet: The human pancreatic duct epithelial cell line (HPDE6-C7) and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were obtained from ATCC.

Techniques: Activity Assay, Knockdown, Staining, Fluorescence, Microscopy, Over Expression, Two Tailed Test, Control

Circ_0041150 targets and regulates miR-1178-3p in PAAD cells. A , B qRT-PCR analysis compared the expression levels of miR-1178-3p, miR-1184, miR-188-3p, and miR-766 in BxPC-3 and PANC-1 cells transfected with sh-circ_0041150#1 or p-circ_0041150 to controls ( n = 4). C Bioinformatics analysis predicted binding site sharing between circ_0041150 and miR-1178-3p, with wild-type and mutated sequences highlighted, with mutation site in red. D , E FISH analysis confirmed co-localization of circ_0041150 (red) and miR-1178-3p (green) in BxPC-3 (D) and PANC-1 (E) cells, with nuclei counterstained with DAPI (blue) (20×; scale bar = 10 μm). F , G Luciferase reporter assays demonstrated altered activity in BxPC-3 (F) and PANC-1 (G) cells transfected with wild-type (WT) or mutant (MUT) circ_0041150 reporters alongside miR-1178-3p mimic or control mimic ( n = 4). H , I RNA pull-down assays followed by qPCR detected circ_0041150 enrichment in BxPC-3 (H) and PANC-1 (I) cell lysates using biotin-labeled miR-1178-3p or control probes ( n = 4). Controls include: (1) bio-NC probe (negative control, a non-specific biotin-labeled miRNA probe with no homology to miR-1178-3p); (2) bio-miR-1178-3p probe (MUT) (mutant control, biotin-labeled miR-1178-3p probe with a mutated binding site for AADAC mRNA, used to verify the specificity of the interaction between miR-1178-3p and AADAC mRNA). J , K AGO2-RIP followed by qPCR assessed circ_0041150 enrichment in BxPC-3 (J) and PANC-1 (K) cells transfected with miR-1178-3p mimic or control mimic ( n = 4). Controls include: (1) Antibody control: Normal rabbit IgG (the IgG subgroup under each transfection condition), which is paired with anti-AGO2 antibody in every transfection group, serving to exclude non-specific immunoprecipitation signals irrelevant to AGO2; (2) Transfection control: control mimic (a non-specific miRNA mimic with no homology to miR-1178-3p, corresponding to the control mimic group in the assay), used to eliminate non-specific effects caused by miRNA mimic transfection itself (instead of the specific function of miR-1178-3p). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Journal: Discover Oncology

Article Title: Circ_0041150 inhibits proliferation of pancreatic adenocarcinoma cells by regulating triglyceride accumulation via the miR-1178-3p/AADAC axis

doi: 10.1007/s12672-026-04577-1

Figure Lengend Snippet: Circ_0041150 targets and regulates miR-1178-3p in PAAD cells. A , B qRT-PCR analysis compared the expression levels of miR-1178-3p, miR-1184, miR-188-3p, and miR-766 in BxPC-3 and PANC-1 cells transfected with sh-circ_0041150#1 or p-circ_0041150 to controls ( n = 4). C Bioinformatics analysis predicted binding site sharing between circ_0041150 and miR-1178-3p, with wild-type and mutated sequences highlighted, with mutation site in red. D , E FISH analysis confirmed co-localization of circ_0041150 (red) and miR-1178-3p (green) in BxPC-3 (D) and PANC-1 (E) cells, with nuclei counterstained with DAPI (blue) (20×; scale bar = 10 μm). F , G Luciferase reporter assays demonstrated altered activity in BxPC-3 (F) and PANC-1 (G) cells transfected with wild-type (WT) or mutant (MUT) circ_0041150 reporters alongside miR-1178-3p mimic or control mimic ( n = 4). H , I RNA pull-down assays followed by qPCR detected circ_0041150 enrichment in BxPC-3 (H) and PANC-1 (I) cell lysates using biotin-labeled miR-1178-3p or control probes ( n = 4). Controls include: (1) bio-NC probe (negative control, a non-specific biotin-labeled miRNA probe with no homology to miR-1178-3p); (2) bio-miR-1178-3p probe (MUT) (mutant control, biotin-labeled miR-1178-3p probe with a mutated binding site for AADAC mRNA, used to verify the specificity of the interaction between miR-1178-3p and AADAC mRNA). J , K AGO2-RIP followed by qPCR assessed circ_0041150 enrichment in BxPC-3 (J) and PANC-1 (K) cells transfected with miR-1178-3p mimic or control mimic ( n = 4). Controls include: (1) Antibody control: Normal rabbit IgG (the IgG subgroup under each transfection condition), which is paired with anti-AGO2 antibody in every transfection group, serving to exclude non-specific immunoprecipitation signals irrelevant to AGO2; (2) Transfection control: control mimic (a non-specific miRNA mimic with no homology to miR-1178-3p, corresponding to the control mimic group in the assay), used to eliminate non-specific effects caused by miRNA mimic transfection itself (instead of the specific function of miR-1178-3p). Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Article Snippet: The human pancreatic duct epithelial cell line (HPDE6-C7) and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were obtained from ATCC.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Binding Assay, Mutagenesis, Luciferase, Activity Assay, Control, Labeling, Negative Control, Immunoprecipitation, Two Tailed Test

AADAC is a direct target of miR-1178-3p in PAAD cells. A Potential binding site overlap between the 3’ UTR of AADAC and miR-1178-3p was revealed by bioinformatics analysis. B , C Luciferase activity was assessed in reporter plasmids containing wild-type and mutant AADAC 3’ UTR sequences co-transfected with miR-1178-3p mimic or negative control in BxPC-3 (B) and PANC-1 (C) cells ( n = 4). D , E AADAC mRNA levels were quantified in pulldown products from cell lysates of BxPC-3 (D) and PANC-1 (E) cells incubated with biotin-labeled miR-1178-3p or control probes ( n = 4). F , G AADAC mRNA levels were detected in BxPC-3 (F) and PANC-1 (G) cells transfected with miR-1178-3p mimic or control mimic ( n = 4). H , I miR-1178-3p and AADAC mRNA expressions were detected in BxPC-3 cells transfected with miR-1178-3p mimic or control mimic ( n = 4). J Following the relevant transfections, AADAC protein expression levels in BxPC-3 cells were evaluated by Western blot ( n = 3). Lane 1, control mimic; Lane 2, miR-1178-3p mimic. K , L miR-1178-3p and AADAC mRNA expressions were also detected in PANC-1 cells transfected with miR-1178-3p inhibitor or miRNA inhibitor-NC ( n = 4). M Following the relevant transfections, AADAC protein expression levels in PANC-1 cells were evaluated by Western blot ( n = 3). Lane 1, miRNA inhibitor-NC; Lane 2, miR-1178-3p inhibitor. Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Journal: Discover Oncology

Article Title: Circ_0041150 inhibits proliferation of pancreatic adenocarcinoma cells by regulating triglyceride accumulation via the miR-1178-3p/AADAC axis

doi: 10.1007/s12672-026-04577-1

Figure Lengend Snippet: AADAC is a direct target of miR-1178-3p in PAAD cells. A Potential binding site overlap between the 3’ UTR of AADAC and miR-1178-3p was revealed by bioinformatics analysis. B , C Luciferase activity was assessed in reporter plasmids containing wild-type and mutant AADAC 3’ UTR sequences co-transfected with miR-1178-3p mimic or negative control in BxPC-3 (B) and PANC-1 (C) cells ( n = 4). D , E AADAC mRNA levels were quantified in pulldown products from cell lysates of BxPC-3 (D) and PANC-1 (E) cells incubated with biotin-labeled miR-1178-3p or control probes ( n = 4). F , G AADAC mRNA levels were detected in BxPC-3 (F) and PANC-1 (G) cells transfected with miR-1178-3p mimic or control mimic ( n = 4). H , I miR-1178-3p and AADAC mRNA expressions were detected in BxPC-3 cells transfected with miR-1178-3p mimic or control mimic ( n = 4). J Following the relevant transfections, AADAC protein expression levels in BxPC-3 cells were evaluated by Western blot ( n = 3). Lane 1, control mimic; Lane 2, miR-1178-3p mimic. K , L miR-1178-3p and AADAC mRNA expressions were also detected in PANC-1 cells transfected with miR-1178-3p inhibitor or miRNA inhibitor-NC ( n = 4). M Following the relevant transfections, AADAC protein expression levels in PANC-1 cells were evaluated by Western blot ( n = 3). Lane 1, miRNA inhibitor-NC; Lane 2, miR-1178-3p inhibitor. Statistical significance was assessed using two-tailed unpaired Student’s t-test or two-way ANOVA. * P < 0.05 and ** P < 0.01 vs. control

Article Snippet: The human pancreatic duct epithelial cell line (HPDE6-C7) and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were obtained from ATCC.

Techniques: Binding Assay, Luciferase, Activity Assay, Mutagenesis, Transfection, Negative Control, Incubation, Labeling, Control, Expressing, Western Blot, Two Tailed Test

A schematic model summarizes the study results. In the cytoplasm, circ_0041150 acts as a sponge for miR-1178-3p, preventing its binding to the 3’ UTR of AADAC mRNA, thus increasing AADAC expression, inhibiting TG accumulation and reducing PAAD cell proliferation. Conversely, without circ_0041150, miR-1178-3p binds to AADAC mRNA, inhibiting its expression, promoting TG accumulation and enhancing PAAD cell proliferation

Journal: Discover Oncology

Article Title: Circ_0041150 inhibits proliferation of pancreatic adenocarcinoma cells by regulating triglyceride accumulation via the miR-1178-3p/AADAC axis

doi: 10.1007/s12672-026-04577-1

Figure Lengend Snippet: A schematic model summarizes the study results. In the cytoplasm, circ_0041150 acts as a sponge for miR-1178-3p, preventing its binding to the 3’ UTR of AADAC mRNA, thus increasing AADAC expression, inhibiting TG accumulation and reducing PAAD cell proliferation. Conversely, without circ_0041150, miR-1178-3p binds to AADAC mRNA, inhibiting its expression, promoting TG accumulation and enhancing PAAD cell proliferation

Article Snippet: The human pancreatic duct epithelial cell line (HPDE6-C7) and PAAD cell lines (Capan-2, BxPC-3, AsPC-1, PANC-1) were obtained from ATCC.

Techniques: Binding Assay, Expressing